期刊论文

DOUBLE EFFECT OF COLCHICINE TREATMENT ON EXPLANT PROTOCORM OF Hemerocallis citrina BAR

中文

湖南农业大学学报(自然科学版)

1007-1032

1991

02

158-165

10.13331/j.cnki.jhau.1991.02.012

本校为第一机构

将黄花菜叶片、花葶、花梗外植体进行组织培养,诱导获得的愈伤组织,分割、继代培养于6BA2mg/1的MS培养基上,逐步形成圆球茎。在无菌状态下,以浓度0.05%的秋水仙素浸泡、棉花覆盖,或者用浸有加入2%二甲基亚砜的0.02%～0.05%秋水仙素溶液的棉花覆盖圆球茎,经24h处理后,除获得黄花菜多倍体试管苗外,还获得以下结果:(1)来源于叶片、花葶的圆球茎的多倍体苗染色体数目比较稳定(2n=4x=44),而来源于花梗的圆球茎的多倍体苗染色体数目类型较多:(2)用多倍体试管苗叶片培养形成的圆球茎,以及分化多倍体苗的圆球茎继代培养可再生大量的多倍体苗,为快速繁殖黄花菜多倍体苗提供了有效途径;(3)在多倍体苗的形态...

Calli obtained from leaf, scape and pedicle explants of Hemero-callis ciurina Bar in vitro formed protocorms Progressively after they were cutup and subcultured on MS medium with 2.0mg/16 BA.Protocorms were treat-cd with colchicine under aseptic condition. The results were better when theprotocorms Were soaked in 0.05% colchcinc and then covered with cotton or justcovered under cotton with 0.05% colchicine or 2.0% dimethyl sulfoxide and0.02%-0.05% colchicine for 24 hours. Besides getting polyploid buds of Hemc-rocallis citrina Bar, the following results were obtained:1/The chromosomenumber of ...