期刊论文

THE EFFECT OF HELMINTHOSPORAL,A PATHOTOXIN PRODUCED BY Bipolaris sorokiniana,ON THE ACTIVITY AND ISOZYME PATTERN OF SUPEROXIDE DISMUTASE(SOD)IN RESISTANT AND SUSCEPTIBLE WHEAT

中文

湖南农业大学学报(自然科学版)

1007-1032

1993

02

162-170

10.13331/j.cnki.jhau.1993.02.010

本校为第一机构

感病品种沈免85-14黄化苗用根腐毒素处理6,12,24h 后,相对酶活随毒素浓度升高而明显下降;抗病品种Jup73Atd“S”用中等浓度毒素处理的,除处理3h 之外,其余的相对酶活性均升高.以处理12h 达高峰,处理24h 后高浓度有抑制作用而低浓度有激活作用。旗叶圆片、无细胞提取液和线粒体试验呈现了类似的规律.电泳结果表明,根腐毒素对感病品种黄化苗的3条酶带(a,b,c)均有抑制作用,而对抗病品种Jup73Atd“S”这3条酶带均有激活作用,a,c 带的变化大于b 带.旗叶接菌后3d,Jup73Atd“S”5条带均激活,其中a,b_2,b_3,c 带更明显;中间型品种龙辐3号各带均受抑制,而以b_3带受抑最重.

Changes in the activity and isozyme pattern of superoxide dismutase(SOD)were investigated in different wheat organs and mitochondrial fractions treated withhelminthosporal or inoculated with Bipolaris sorokiniana,by using the methods of reductionof nitroblue tetrazolium(NBT)and discontinuous polyacrylamide gel electrophoresis(PAGE),respectively.In the etiolated seedling of susceptible cv.Shenmian 85-14,the relativeactivity(RA)of SOD remarkably decreased with the increasing concentrations of the toxinafter 6.12 h and 24 hour-treatments.In contra...