In this paper,we have established the analytical methods(including identifica-tion and quantity)of DHA and EPA in fish oil and PUFA through the analysis of fatty acidcomposition of fish oil and PUFA. Refined clear fish oils in yellowish orange color were ob-tained through degumming,neutrialization and bleaching, and the odour of refined fish oilswere improved greatly.The contents of DHA, EPA in refined fish o...