期刊论文

中文

湖南农业大学学报(自然科学版)

1007-1032

1994

06

550-554

10.13331/j.cnki.jhau.1994.06.009

湖南省教委资助的重点课题；

本校为第一机构

以福鼎大白无性系茶树大田种子胚为材料，切取子叶、胚芽、胚轴和胚根进行培养，诱导形成愈伤组织。选用较致密的愈伤组织切块与带ＮＰＴⅡ和ＧＵＳ基因Ｔｉ质粒的根癌农杆菌共培养，通过卡那霉素抗性选择，得到了抗性愈伤组织并得到了芽的分化。抗性愈伤组织的诱导率在５％左右。共培过程中发现茶树愈伤组织能较强烈地促进农杆菌生长，表现在农杆菌在茶树愈伤组织周围的聚结。这可能是由于茶树多酚类含量较高，而通常酚基复合物有利于农杆菌对植物细胞的附着和转化。

With the materia1 of embryo from field seeds of Thea sinensis L.var.Fud-ingdabai asexual reproduction lines,different explants of cotyledon,plumule,epicotyle,radicle were cultured in vitro to induce callus formation.The cutting pieces of the tighten callus were then co-cultured with Agrobacterium tumefaciens which carries NPT Ⅱ,GUS geiies on their Ti plasmid.After selecting with kanamycin,resistant callus were obtained and resistant buds induced.The inducing rate of resistant callus was about 5%,It was found that the callus fa-cilitated the ba...