期刊论文

STUDIES ON CATALYTIC ACTIVITY OF DNA MOLECULES Ⅲ.THE CATALYTIC FUNCTION OF POLYRIBOTIDE

中文

湖南农业大学学报(自然科学版)

1007-1032

1996

22

2

103-105

10.13331/j.cnki.jhau.1996.02.001

湖南省教委高校青年骨干教师培养基金；

本校为第一机构

以多聚腺苷酸（ｐｏｌｙＡ），多聚尿苷酸（ｐｏｌｙＵ）及其混合复性的ｐｏｌｙＡ·ｐｏｌｙＵ与乙酸－α－萘酯及坚牢蓝ＲＲ盐混合保温，研究了多聚核苷酸催化乙酸－α－萘酯水解的作用，结果表明：由ｐｏｌｙＡ·ｐｏｌｙＵ组成的双链ＲＮＡ具有与ＤＮＡ分子相似的催化活性，ｐｏｌｙＡ这一单链多聚物也能起催化作用，ｐｏｌｙＵ则没有这一功能。据此认为，核酸分子的催化活性与核糖２＇－位脱氧与否无关，而碱基的堆积为素酯分子的疏水奈环基团提供足够的疏水空间，则是催化活性所必须的。双链ＤＮＡ及双链ＲＮＡ都能满足这一条件，ｐｏｌｙＡ中嘌呤碱基堆积则刚能容纳下萘环，所以均能催化...

The color reaction of catalyzing α-Naphthylacetate to hydrolyze polyribotidepoly(A),poly(U)and their renaturated mixture poly(A)poly(U)incubated together withα-Naphylacetate and fast blue RR salt was studied.The result showed that the annealed poly(A)· poly(U) and the single strand polymers of Adenine were able to hydrolyze α-Naphthy-lacetate but poly(U)was not.It suggested that no matter that the2'-OH of riboes was deox-idated or not,its catalvtic ability was not affected,but the pattern of the bases stacking alongthe strand which provided...