期刊论文

中文

湖南农业大学学报(自然科学版)

1007-1032

1996

22

3

239-244

10.13331/j.cnki.jhau.1996.03.006

湖南省高校博士点基金

本校为第一机构

以苎麻子叶、下胚轴为外植体, 在含高钾、磷、硝态氮、肌醇、盐酸硫胺素及低钙、低氨态氮, 以葡萄糖为碳源的CXW培养基上最易获得愈伤组织; 激素组合为0.5mg/L CPA(或2, 4-D)和0.05mg/L BR; 培养温度为28℃。愈伤组织在初代和继代培养中存在丰富的多样性, 但在附加0.1mg/L CPA、0.5mg/L NAA。1.5mg/L ZT的CXW培养基上能获得多样性相对较小、质地结构较好的愈伤组织, 并在附加0.1mg/L CPA、0.5mg/L NAA、1.5mg/L ZT、2mg/L Met、3g/L YE的CXW培养基上首次获得了少数畸形的胚状体幼苗。

Thirty-one ramie varieties were used to have a systematic study on calli induc-tion.The results showed that cotyledon and hypocotyl were suitable explants which producedcallus easily on CXW basic medium containing high K,P,NO3,inositol thiamine,but low Caand;with hormone combination being 0.5mg/L CPA(or 2,4-D)and 0.05 mg/L BR,temperature being 28℃.Calli polymorphism occurred cduring induction eulture and subcul-ture,However,the polymorphism expressed relatively little when subcultured on CXW medi-um containing 0.1mg/L CPA,0.5mg/L NAA and 1.5 m...