期刊论文

中文

湖南农业大学学报(自然科学版)

1007-1032

1996

22

6

516-523

10.13331/j.cnki.jhau.1996.06.003

国家教委基金;国外基金;国家自然科学基金

本校为第一机构

通过溶剂系统的选择，建立了用硅胶柱层析、薄层层析和反相高效液相色谱分离纯化稻叶中ＭＧＤＧ和ＤＧＤＧ的方法．该方法也适用于其它植物叶中半乳糖脂的分离纯化．硅胶柱层析在除去大量色素和中性脂方面效率较高，反相ＨＰＬＣ适于制备少量的标准ＭＧＤＧ和ＤＧＤＧ纯品．通过专一性脂肪酶水解和碱水解，并经ＨＰＬＣ分析，水稻ＭＧＤＧ和ＤＧＤＧ中α－亚麻酸（１８３脂肪酸）的含量占不饱和脂肪酸总量的９６％以上，不含有１６３脂肪酸，由此可以确定水稻属于１８３植物．在ＭＧＤＧ的甘油部分Ｃ－１位上总是连接α－亚麻酸，而Ｃ－２位上除大部分连接α－亚麻酸外，还连接有少量的１８２，...

A method of extraction and purification of monogalactosyldiglyceride (MGDG) and digalactosyldiglyceride (DGDG) in rice leaves was gained based on silica gel column chromatography,TLC and reversed phase HPLC.Most pigments and neutral lipids could be removed from total lipids with silica gel column chromotography,but reversed phase HPLC was only suitable for the treatment of a small amount of pured MGDG and DGDG preparation.The results of HPLC analysis showed that over 96% unsaturated fatty acid was α linolenic acid (18∶3),and no 16∶3 fatty ac...