The 1dayaged and 12daysaged chicks which had high level of ND maternal antibody were inoculated with four times and defined dosage of NDⅡ strain vaccine,respectively. They were intratracheally inoculated with the suspension of pathogenical Escherichia coli at the third day of postvaccination. The morbidity,numbers of WBC,heterophil leucocytes and ANAE+ cells,percentage of transformed lymphoblastic cells and serous ND HI antibody titers were determined in order to evaluate the mutual effects of the prior ND vaccination and artific...