期刊论文

中文

湖南农业大学学报(自然科学版)

1007-1032

1998

24

6

436-441

10.13331/j.cnki.jhau.1998.06.004

袁隆平研究基金,湖南省教委资助；

本校为其他机构

为探索无融合生殖基因转移途径，用穗茎注射法将无融合生殖大黍总ＤＮＡ导入水稻品系桂９９，获得１雌不育变异，其有性胚囊败育方式与大黍相似，为揭示穗茎注射的外源ＤＮＡ在体内的运输途径，用显微放射自显影技术进行了研究。结果表明，外源ＤＮＡ能经维管束的导管被运转到花器官中。

In this experiment,the total DNA of Panicum maximum is injected into the stems of rice (Oryza Sativa L.) when the rice is in its early microspore developing stage.As a result,a female sterile variant (FSV 1) is identified from the recipient Gui 99 (a restorer line).The variants functional megaspore degenerates as the pattern in Panicum maximum.This aims at finding out the apomixis genes transfering way in rice.In the course of this experiment,a micro radioautographic technology is introduced into the study on the transportation ...