期刊论文

中文

湖南农业大学学报(自然科学版)

1007-1032

1999

25

2

87-91

10.3321/j.issn:1007-1032.1999.02.001

(01-941-06):湖南省科技厅科研项目

本校为第一机构

为了选育高产、优质、多抗的水稻新品种,采用花粉管通道法,将密穗高粱总DNA导入水稻晚粳品种鄂宜105,从D1至D5代变异材料中选育出DH3,DH4,DH5等多个具有高光效、高产和优质的新品系.经随机扩增多态DNA技术对供体、受体、后代进行分子验证,后代中出现了在供体中存在而在受体中不存在的泳带.解剖学观察发现了后代剑叶、穗颈及穗基的输导组织比受体发达,维管束数目比受体增加10%～30%.后代DH3,DH4的光合速率亦显著高于受体.在形态解剖、光合特性和分子水平上进一步证明了高粱DNA片段在受体中的表达和稳定遗传.对DH3,DH4,DH5等品系进行生产示范,增产效果显著,单产高达7500 kg/hm2.DH5已于1999年2月通...

To select high yield,high quality and high disease resistance rice variety,sorghum total DNA was introduced into later season rice Eyi 105 through rice pollen tube in this experiment. More than 10 desirable lines (DH series) were selected from the variant plants from D 1 to D 5 generations. RAPD analysis showed that some electrophoresis bands were found in the selected lines which was also found in the sorghum supplier but not in Eyi 105 acceptor. Anatomical observation found that the flag leaf and the bottom stem of ear in DH series had about ...