期刊论文

中文

湖南农业大学学报(自然科学版)

1007-1032

2000

26

6

432-435

10.3321/j.issn:1007-1032.2000.06.008

湖南省自然科学基金资助项目 !（93- 14）；

本校为第一机构

为了建立基因枪介导的西瓜遗传转化系统,以西瓜顶芽为受体,pBI121为供体,采用正交试验设计,研究了基因枪的扩散腔类型、轰击次数、受体的预培养和高渗处理对转化率的影响。经方差分析与差异显著性测验,预培养时间与轰击次数对转化率影响显著,而扩散腔类型和甘露醇处理质量浓度对转化率的影响不显著。经组织化学检测,有33.3%抗Kan试管苗为GUS阳性。

Effects on the transformation rate of terminal buds of watermelon by gene gun's expanding cavity,time of bombment,pre culture and high seeping treatment of acceptor were studied by using the terminal buds of watermelon as acceptor,plasmid pBI121 as donor and orthogonal design to establish the system of genetic transformation of watermelon.The results show that the time of pre culture and number of bombment impose a striking effects on transformation rate,while the type of expanding cavity and the concentration of Mannitol affect the transformation insignificantly.There are 33 3% anti Kan plant...