期刊论文

中文

湖南农业大学学报(自然科学版)

1007-1032

2000

26

2

110-112

10.3321/j.issn:1007-1032.2000.02.011

湖南省教委资助项目! （93- 1 1 1 ）；

本校为第一机构

为探讨茶树茎段培养中胚性状态的调控及其分化机理 ,以种子苗和成龄茶树嫩梢为材料 ,研究了培养基、培养时间对茎分化及分化状态保持的影响 .结果表明 ,茶树茎分化需要 2个月以上的诱导培养过程 .茎段产生分化后 ,在不同组分的培养基上交叉培养 ,维持分化能力的时间比在单一培养基上的长 .具有少量母体组织的不定芽苗在其基部分化了体细胞胚、不定芽 ,并在其叶上分化了体细胞胚 .不定芽苗在初代培养中 ,其切口处易诱导出非胚性愈伤组织 ,切除非胚性愈伤组织后 ,诱导出了胚性愈伤组织

In order to inquire into the mechanism of regulation and control of embryogenic state in stem of tea plant culture,the present paper studied the effect of media and culture time on differentiation and maintenance of differetiation state with the stem sections of seedling plant and shoot of adult plant. The results indicated that:inducement culture for more than 2 months was necessary on differentiation in the stems of tea plant culture. When differentiation was appearing in the stem,the maintenance time of plant regeneration capacity of the stem cultured alternately on mediums with different c...