期刊论文

中文

湖南农业大学学报(自然科学版)

1007-1032

2001

27

6

476-478

10.3321/j.issn:1007-1032.2001.06.019

湖南省教育厅科研项目

本校为第一机构

为探寻茶树外源DNA导入的最佳材料,以茶树东湖早品种的种子胚和不同组织等为材料,研究了植物激素(BA,IBA,GA3)对畸形叶发生,不定芽、体细胞胚分化的影响.结果表明,在一定的激素组合条件下,畸形叶仅发生在种子胚第1,2次生长的第1～3叶位,不定芽分化率高低顺序依次为畸形叶、具有腋芽组织的叶柄、子叶柄;丛芽的分化率,子叶柄高于叶柄;子叶柄的不定胚分化率为35.3%,顶芽为6.0%.

To seek the best material of DNA introduction, the effects of plant growth regulators(BA,IBA,GA 3) on appearance of abnormal leaf,differentiation of adventitious bud and somatic embryo were studied with embryo and different tissues,buds of tuber-plant of Donghuzao tea plant. The results show that when plant growth regulators with various combinations are proper, abnormal leaves only appear on the 1st～3rd leaf position of the first and the second growth in whole embryo. Differentiation rate of adventitious bud decreases in the following order: ...