期刊论文

中文

湖南农业大学学报(自然科学版)

1007-1032

2001

27

1

60-62

10.3321/j.issn:1007-1032.2001.01.018

湖南省教委资助项目!(97B0 2 5 )；

本校为第一机构

为探明单宁酶应用于生产的条件,从黑曲霉发酵液中分离提纯了单宁酶,经过(NH4)2SO4分步沉淀、DEAE-纤维素离子交换层析和Sephadex G-150凝胶层析等3步纯化,得到电泳纯的单宁酶.用SDS-PAGE法测得该酶有2种亚基,相对分子质量分别为7.59×104,6.03×104.该酶为糖蛋白,蛋白质含量为72.5%,糖含量为12.3%.该酶的最适pH为5,最适温度为40℃.

To establish a method of purifying tannase and exploit the application conditions of it , the tannase of Aspergillus niger No.1 was separated and purified by (NH 4) 2SO 4 fractional precipitation, DEAE cellulose ion exchange chromatography and gel filtration. It was determined by SDS PAGE that the tannase has two kinds of subunits;their molecular weight is 75 9 kD and 60 3 kD respectively, the content of protein is 72 5% and the content of suger is 12 3%. Its...