期刊论文

中文

湖南农业大学学报(自然科学版)

1007-1032

2001

27

5

410-414

10.3321/j.issn:1007-1032.2001.05.025

国家科技部基础性项目 （ 1999- 2 2 5 ）资助；

本校为第一机构

微卫星作为第二代分子遗传标记,已经在很多领域得到广泛应用.微卫星位点的获得有两条常用技术途径:1)利用已发现的微卫星位点寻找新的位点;2)用经典分子生物学方法克隆特定基因组的微卫星位点.微卫星分析的一般步骤包括:DNA的提取,PCR扩增,扩增产物及大小的检测和数据分析.DNA芯片技术的出现,为微卫星技术的应用提供了更广阔的前景.在动物遗传多样性研究中,可以通过微卫星技术进行种间、种内、群体之间、甚至是个体之间的亲缘关系分析包括亲子鉴定等.

As one of the second generation of molecular genetic markers,microsatellites have a wide range of applications which previously were difficult to address experimentally. Microsatellites loci could be obtained in two ways:1 ) the simplest means of identifying microsatellites is by using described sequences;2 ) the new microsatellite loci have been obtained by standard molecular cloning. The procedure of microsatellites analysis includes the following steps:DNA extraction,PCR reactions,visualizing microsatellite products and size standards,bio- information analysis of microsatellite data.With th...