期刊论文

中文

湖南农业大学学报(自然科学版)

1007-1032

2003

29

5

412-414

10.3321/j.issn:1007-1032.2003.05.014

国家“8 6 3”计划资助项目 （ 10 1- 0 6 - 0 2 - 0 3）；

本校为第一机构

采用显微注射法将人α干扰素抗病基因转移到草鱼受精卵中，获得转基因群体，通过PCR及Southern杂交对转基因草鱼进行分子水平的检测，以进一步验证外源人α-干扰素是否整合人草鱼基因组。结果表明，外源人α-干扰素抗病基因已整合人草鱼基因组中采用腹腔注射法，对转基因鱼进行草鱼出血病病毒接种攻毒试验，结果显示转基因草鱼比对照组的抗病毒能力有显著提高。

A group of transgenic grass carps were obtained by microinjecting of hu IFN α gene into grass carps zygotes. PCR analysis and Southern blot were carried out to verified transgenic grass carps.Results show that the exotic disease resistance gene hu IFN α is integrated into the genome of transgenic grass carps. The ability to resist hemorrhage virus (GCHV) of transgenic group is obviously higher than common grass carp in the challenge ...