期刊论文

中文

湖南农业大学学报(自然科学版)

1007-1032

2004

30

6

513-515

10.3321/j.issn:1007-1032.2004.06.005

湖南省教育厅重点资助项目（99B05）；

本校为第一机构

为探讨水稻灌浆期间与籽粒充实相关蛋白表达的变化,建立了一套适于水稻种子胚乳蛋白双向电泳分析技术,探讨了胚乳蛋白的提取、纯化以及双向电泳的一些关键技术.结果表明,通过乙醇铵沉淀、高压等电聚焦和两步平衡等方法可以获得较好的分析结果,获得胚乳蛋白斑点数约350个,等电点范围在3.0～9.0,其中大部分集中在4.0～7.0.

In order to research relative proteins with grain plumpness during filling stage of rice,one serial of 2-D electrophoresis which be suitable to endosperm proteins of rice seed was set up.It was discussed on the extraction, purification of the endosperm proteins and some key technologies of 2-D electrophoresis.The results were showed that, by precipitating with ammonium ethanol isoelectric focusing under high-voltage and two-step equilibrium,about 350 protein spots that the isoelectric point ranges from 3.0 to 9.0 were found...