期刊论文

中文

湖南农业大学学报(自然科学版)

1007-1032

2004

30

5

433-436

10.3321/j.issn:1007-1032.2004.05.009

湖南省教育厅资助项目（02C069）；

本校为第一机构

为建立屋顶长生花快速繁殖体系,以希腊引种的屋顶长生花肉质叶为外植体,通过组织培养,对影响其愈伤组织诱导、玻璃化苗现象及根、芽分化的几个主要因素进行了研究.结果表明,不同种类激素组合对其愈伤组织形成、芽分化以及根的形成有不同的影响.以MS+2,4-D 2.0 mg/L+6-BA 2.0 mg/L为最适诱导愈伤组织培养基;以MS+6-BA 2.0 mg/L+NAA 0.2 mg/L+GA3 0.4 mg/L为最适分化培养基;用于继代增殖的培养基,6-BA的质量浓度为0.3(4 mg/L;生根以1/2MS+ NAA 0.2 mg/L+IAA 1.0 mg/L+0.3%活性炭为宜.采用增加光照度、降低培养温度、增加培养基中蔗糖和琼脂质量分数以及加入青霉素等措施能较有效地防止玻璃化苗现象...

To establish the technical system of rapid propagation of Sempervivum tectorum with its leaves used as explants this experiment is an attempt to study the ways of shoot regeneration.The results of current study show that the combination of different kinds and concentration of hormone affects the formation of callus,shoot induction and rooting of plantlet.(1) The optimum medium for callus is MS+2,4-D 2.0 mg/L+6-BA 2.0 mg/L;(2) The optimum medium for differentiation is MS+BA 2.0 mg/L+NAA 0.2 mg/L+GA3 0.4 mg/L;(3) The different concentration of 6-BA has litter effect on shoot multiplication;(4) T...