This article deals with isolation from fermenting-fruit of pineapple and orange.Through identified as LAB; the effects of artificial incubation on MRS-CHOL,carry on the ability of degrading-cholesterol test, under 38 ℃ constant temperature 3 day. The results show that the 2 strains have the capability of degrading-cholesterol, with the degradation rate of the cholesterol increasing during different time and different quantity of incubation and low-cholesterol density among the culture .With the decrease of high-cholesterol density,2 strains po...