期刊论文

中文

湖南农业大学学报(自然科学版)

1007-1032

2005

31

6

627-630,655

10.3321/j.issn:1007-1032.2005.06.011

国家转基因专项资助项目（YJ04-B-02）； 湖南省科技厅重大科技专项（04NK1005）；

本校为第一机构

为改良柑桔品种,以普通溆浦甜橙(Citrus sinensis cv Xupu)实生苗上胚轴为外植体,建立了农杆菌介导的遗传转化体系,并把chit42基因和phyB基因导入其中.上胚轴经农杆菌工程菌液浸染后,在共培培养基(MS+3mg/L BA)上培养3 d,然后分别转移到含有100 mg/L Kan(卡那霉素,Kanamycin)和500 mg/L Cef(头孢噻肟霉素,Cefotaxime)的选择培养基上培养14 d后,上胚轴开始分化抗性芽,chit42基因和phyB基因的抗性芽再生率分别为27.8%和35.6%.将抗性芽在筛选培养基上培养30 d后用茎尖微芽嫁接成苗.目前,嫁接苗在温室中生长良好,经PCR鉴定为转化植株.

Seedling epicotyl （intemodal stem） segments of ‘Xupu＇ sweet orange（Citrus sinensis L.Osb.）were used as explants to establish Agrobacterium-mediated transformation system with chit42 and phyB genes. The results showed that transformed buds could be obtained after the epicotyl segments were cultured on co-culture medium （MS＋3 mg/L BA） for 3 days and then on selective medium with 100 mg/L Kanamycin and 500 mg/L Cefotaxime for 2 weeks. The transformation efficency of Sweet orange with the chit42 and phyB genes reached 27.8% and 35.6%, resp...