期刊论文

中文

湖南农业大学学报(自然科学版)

1007-1032

2005

31

6

623-626

10.3321/j.issn:1007-1032.2005.06.010

国家转基因专项资助项目（YJ04-B-02）； 湖南省科技厅重大科技专项（04NK1005）；

本校为第一机构

为建立大红甜橙的再生及遗传转化体系,提高转化芽的成株率,以30 d龄的大红甜橙(Citrus sinensisOsbeck)实生苗上胚轴为材料,导入rolB基因,进行遗传转化研究.结果表明:MS+3.0 mg/L 6-BA培养基诱导不定芽的效果最好,达95%以上.遗传转化时,采用培养基Ⅱ(MS+3mg/L6-BA+200μmol/LAs)+培养基Ⅳ(MT+3 mg/L 6-BA+50 mg/L Kan+500 mg/L Cef)组合的抗性芽诱导最快,诱导率为51%.用微芽嫁接的方法,将抗性芽嫁接到卡里佐枳橙上,成苗良好,已获得19株转rolB基因植株,盆栽在温室中生长良好.

Internodal stem segments from 30 d old seedlings of ‘Dahong＇ sweet orange（Citrus sinensis Osbeck） were cultured on bud induction media, to study on the genetic transformation in Citrus with rolB gene. The best medium for induction was MS＋3.0 mg/L 6-BA, which led to 95% of explants producing at least one bud, so this medium could be considered as the best for bud induction in plant regeneration. The co-culture medium Ⅱ （MS＋3 mg/L 6-BA＋200 μmol/L As）＋selective medium Ⅳ （MT＋3 mg/L 6-BA＋50 mg/L Kan＋500 mg/L Cef） were the suitable ...