期刊论文

中文

湖南农业大学学报(自然科学版)

1007-1032

2006

32

6

616-618

10.3321/j.issn:1007-1032.2006.06.012

110200001012:中国烟草总公司科技项目计划

本校为第一机构

为了建立一种快速、准确测定烤烟叶中类胡萝卜素含量的方法,探索了反相高效液相色谱法分离类胡萝卜素的试验条件.结果表明,采用90%丙酮提取和0.1%二丁基羟基甲苯抗氧化剂保护,用乙酸乙酯和90% 含水乙腈溶液作为流动相进行梯度洗脱,可以快速、准确地分离测定烤烟叶中β-胡萝卜素和叶黄素的含量.该法具有良好的线性(相关系数为0.999 1～0.999 8)和重复性(相对平均偏差小于2.21%),平均回收率可达到94.25%,并可有效防止类胡萝卜素氧化,适用于大批量样品的分析检测.

In order to establish a accurate and quicky method of determinating carotinoids content in the flue-cured tobacco leaves, it was probed into the experiment condition on departing and determinating carotinoids content by reversed-phase high performance liquid chromatography（RP-HPLC）. The results made known that extracted by 90% acetone which contain antioxidant of 0.1% dibutylhydroxyl mehtylbenzene（BHT）, gradient washed by acetic ester and 90% acetonitrile, it can be quickly separation and accurately determinating content of β-carotene and ...