期刊论文

Construction of a subtractive cDNA library from the intestinal tissue of grass carp experimentally infected by Aeromonas hydrophUa

中文

湖南农业大学学报(自然科学版)

1007-1032

2007

33

6

718-722

10.13331/j.cnki.jhau.2007.06.028

湖南省科学技术厅项目（湘03NKY1001）； 湖南农业大学动物医学院青年基金项目（SP-0354）；

本校为第一机构

以致病性嗜水气单胞菌（Aeromonas hydrophila）人工感染的1龄草鱼（Ctenopharyngodon idellus）肠道组织为材料，应用抑制性差减杂交技术，构建了肠道组织的消减cDNA文库．以草鱼管家基因β-actin作为差减指标检测文库差减效率达2^10倍，表明感染细菌后某些差异表达基因得到了相应倍数的富集．将获得的cDNA片段连接到A／T载体，并转化大肠杆菌DH5a感受态细胞．

Aeromonas hydrophila is one of the main causative agents resulting in serious infectious diseases of grass carp （Ctenopharyngodon idellus） and other animals. To understand anti-infectious response to bacteria in grass carp, a subtractive cDNA library was constructed from the intestinal tract of grass carp experimentally infected with Aeromonas hydrophila using suppression subtractive hybridization（SSH）. Grass carp β-actin gene was used as internal control to estimate the efficiency of subtractive cDNA. In this library, β-actin was subtrac...