期刊论文

中文

湖南农业大学学报(自然科学版)

1007-1032

2007

33

6

751-753

10.13331/j.cnki.jhau.2007.06.008

湖南农业大学拔尖人才项目（BJ2004）；

本校为第一机构

将紫茎泽兰的乙醇提取物依次用石油醚、氯仿、乙酸乙酯、正丁醇萃取，萃取物对马铃薯晚疫病菌进行抑菌试验．结果表明，石油醚萃取物抑菌效果最好，抑菌率达100%．进一步采用不同梯度的混合有机溶剂对石油醚萃取物进行硅胶柱层析，抑菌试验结果表明，用石油醚∶乙酸乙酯（8∶1）、石油醚∶乙酸乙酯（4∶1）和甲醇试剂洗脱得到的有效物质，其抑菌效果最好，抑菌率达100%．

Extraction of Eupatorium adenophorum Spreng has the higher suppression effects on Phytophthora infestans （Mont.） de Bary. Corresponding extraction was obtained by extraction of Eupatorium adenophorum Spreng with petroleum ether, chloroform, ethyl acetate and normal butyl alcohol, followed by the subsequent steps： determination of the active ingredients of Eupatorium adenophorum Spreng derived from extraction of petroleum ether through the bacteriostasis, reuse of different of gradient mixed organic solvents to its concentration and the silic...