期刊论文

中文

湖南农业大学学报(自然科学版)

1007-1032

2007

33

5

576-579,607

国家转基因植物研究与产业化开发专项（JY04-B-02）； 湖南农业大学人才科学基金（03YJ11）；

本校为第一机构

以大红甜橙的实生苗为试材,研究6-BA与IAA的不同浓度组合和Km不同浓度对大红甜橙不同外植体离体再生的影响和IBA的不同浓度对不定芽生根的影响,建立了高效的大红甜橙遗传转化再生体系.该体系的最佳条件是:1)以实生苗上胚轴为外植体.2)以MS无机盐+100 mg/L肌醇+0.2 mg/LV-B_1+1 mg/LV-B_6+1 mg/L烟酸+3%蔗糖+0.8%琼脂(pH5.8)+3 mg/L 6-BA为不定芽诱导培养基,在26℃黑暗条件下培养7 d后,转移至光/暗周期16/8 h的条件下诱导不定芽的分化(40 d分化率达90%).以1/2MS+3%蔗糖+0.7%琼脂+2.0 mg/L IBA为不定芽的生根培养基(40 d生根率达94.7%).3)筛选遗传转化转化体的Km质量浓度为50 mg/L.

Using epicotyl and hypocotyl pieces and cotyledons of Citrus sinensis Osbeck cv. Dahong seedlings grown in vitro as explants, an efficient regeneration system was established. The optimal conditions were obtained as follows. 1） The epicotyl pieces were used as explants. 2） The optimal adventitious shoot regeneration medium was MS salts ＋100 mg/L inositol ＋0.2 mg/LV-Bl＋l mg/LV-B6＋I mg/L niacin ＋ 3% sucrose＋0.8% agar＋3 mg/L 6-BA, pH5.8. The best response was obtained when the explants were incubated at 26 ℃ for 7 days in darkness, follo...