期刊论文

中文

湖南农业大学学报(自然科学版)

1007-1032

2008

34

2

182-185

湖南省重大科技专项（04NK1005）；

本校为第一机构

为了建立椪柑成年态离体高效再生体系,比较了7种消毒方法的灭菌效果和4种培养基配方,结果表明:改良消毒法能有效降低成年态桠柑节间茎段的污染率(约12.2%)和死亡率(约27.2%),增加外植体的萌芽率(约12.8%)和成功率(约30.6%);MS+BA 1 mg/L+NAA 0.5 mg/L最适合椪柑成年态节间茎段生长,萌芽率达12%;另外,1 cm左右的成年态接穗茎尖嫁接的成活率仅约10%,选用5 mm以下的茎尖可明显提高嫁接成活率,使用1 mm左右茎尖进行微芽嫁接成活率最高,这可能与柑橘接穗越大携带的内生菌越多有关.

Aiming at setting up an efficient regeneration segments of Ponkan （Citrus reticulata Blanco） in vitro, system by direct organogenesis from mature internodal stem seven methods were tested for their sterilization of mature materials, and four regeneration medium were compared. The result indicated that the combination of pre-sampling and post-sampling disinfection （innovation method） was the most effective one, with the rates of the contamination and death reduced to 12.2% and 27.2%, respectively. After sterilization with the innovation meth...