期刊论文

中文

湖南农业大学学报(自然科学版)

1007-1032

2008

34

2

177-181

:国家转基因植物研究 (JY04- B-02):产业化专项基金

本校为第一机构

为建立糖橙的高效遗传转化体系,把无融合生殖基因DefH9-iaaM导入到糖橙中,以获得定向改良目的性状的糖橙新种质.以实生态糖橙节间茎段为材料,建立了糖橙的高效再生体系.结果显示,30 d左右苗龄的实生态糖橙茎段不定芽再生率较高;暗培养3~6 d有利于节间茎段不定芽的分化;不定芽再生的最佳培养基配方为MS附加6-BA 5.0 mg/L,平均每根茎段可以再生3.3个小芽;MS+6-BA 1.0 mg/L+NAA 0.1 mg/L有利于不定芽的增殖;MS附加NAA 0.5 mg/L有利于再生芽的生根.用农杆菌介导法进行转化后,经PCR鉴定已获得6 株转基因株系.

A parthenocarpy gene （DefH9-iaaM） was introduced into Succari to obtain possible seedless transformants, by using internodal stem segments as explants to establish an efficient regeneration and transgenic system. The results indicated that 30-day-old seedlings were the most efficient explants. The co-culture in darkness for 3-6 days gave a rise to good regeneration. The optimum medium for adventitious buds regeneration was MS＋6-BA 5.0 mg/L. And MS medium supplied with 6-BA1.0 mg/L and NAA0.1 mg/L showed the highest bud multiplication rate, 3...