DNAs of sets of samples from 13 cultures which were from 11 different species often infected soybean were extracted. Based on ITS sequences comparison, two PCR primers were designed for Phialophora gregata f.sp.sojae. The specific primers were used in PCR to amplify a 500 bp DNA fragment in isolate of Phialophora gregata. And dilution was up to 4.2 pg/μL, the pathogen also can be detected. Finally, this assay also was successful used to detect the pathogen in inoculated plant. This new method is a powerful tool f...
