期刊论文

中文

湖南农业大学学报(自然科学版)

1007-1032

2008

34

6

719-723

基金项目：湖南省科学技术厅重大专项（湘科技[2005]101号1

本校为其他机构

应用AFLP技术,使用E0/M0预扩引物及5对选扩引物(E41/M42,E41/M55,E42/M34,E43/M42,E44/M33),对以槠叶齐、福鼎大白茶、白毫早、龙井43为父本,湘波绿为母本的混合花粉授粉茶树杂交F1代79个单株进行分析.聚类结果将混合花粉授粉杂交的4个父本完全分开,其F1代79个单株分别归于4个父本所在的类群里;遗传距离为0.085～0.83,表明茶树是高度杂合的,混合花粉授粉杂交后代各单株间的遗传变异大;混合花粉授粉可以提高茶树杂交的可孕性,改变亲本间的亲和力;茶树混合花粉授粉杂交比单一父本杂交F1代的分离度更大.

In this paper, 79 single bushes of the F1 progenies from a mixed-pollen cross（every kind of pollen was mixed equally） in 1980 and their parents （XiangboltixZhuyeqi, Fudingdabaicha, Baihaozao and Longjing43） were used as the experimental materials. They were researched by AFLP molecular markers with the pre-amplification primer pairs E0/M0 and the five selective amplication primer pairs （E41/M42, E41/M55, E42/M34, E43/M42, E44/M33）. The main results were as follows： 1） Based on Nei＇S genetic distances, F1 progenies and their paternals w...