HPLC method for determination of content of phyllanthin in Phyllanthus urinaria L. plant samples was collected from different resourses. The HPLC of UV detection and gradient elution was reviewed. Confirmed separation was carded out on an ODS column and mixture of methanol-water(80 : 20) as the mobile phase was used. The flowing rate was 0.8 mL/min and detection was affected at 230 nm. The experimental results showed that the assay displayed good linearity over the concentration range ...