Solid state fermentation was optimized for improvement ofraw-starch-hydrolysing amylase production by Aspergillus niger AF-1. Potato flour, soybean powder, and FeSO4 were screened out as the most suitable carbon source, nitrogen source and inorganic salt source, respectively. Based on these, screening methodology Plackett-Burman design was used to evaluate the effects of twelve factors related to amylase production and three statistically significant factors soybean powder, temperature, bran were selected. The path of steepest ascent was used t...