期刊论文

中文

湖南农业大学学报(自然科学版)

1007-1032

2009

35

1

88-91,103

10.13331/j.cnki.jhau.2009.01.026

06NK3049:国家科技攻关计划 07A012:湖南省教育厅重点项目 QN0702:湖南农业大学青年项目

本校为第一机构

采用RAPD技术,选用11条有效随机引物研究了南洞庭湖15种野生蚌(圆顶珠蚌、尖锄蚌、圆头楔蚌、鱼尾楔蚌、巨首楔蚌、射线裂脊蚌、球形无齿蚌、勇士尖嵴蚌、扭蚌、杜氏珠蚌、背瘤丽蚌、短褶矛蚌、三巨瘤丽蚌、环带丽蚌、蚶形无齿蚌)之间的遗传多样性,得到RAPD-DNA扩增条带1 775条,片段长度500～1 500 bp,平均每条引物扩增出43.5条带,这些条带构成了南洞庭湖15种野生蚌的遗传多样性图谱.分析表明,属间的遗传距离以裂脊蚌属和尖嵴蚌属间最近,为0.485 7,丽蚌属与其他属间的遗传距离最远,为0.729 2;种间的遗传距离以圆头楔蚌和巨首楔蚌间最小,为0.333 3,背瘤丽蚌与三巨瘤丽蚌及环带丽蚌的遗传距离最大...

RAPD technology was used to study fifteen wild freshwater mussel species in South Dongting Lake, and 11 random primers were selected. 1 775 RAPD bands have been obtained, length between 500 bp and 1 500 bp, its band rate of primer 43.5, these bands have revealed genetic diversity maps of the fifteen wild freshwater mussel species in south dongting lake. Genetic analysis of these mussels indicated, the closest genetic distance among genera is 0.485 7, between Schistodesmus and Acuticosta, the farthest is 0.729 2, between Lamprotula and the others. The closest genetic distance among species is 0...