期刊论文

中文

湖南农业大学学报(自然科学版)

1007-1032

2010

36

5

569-573

10.3724/SP.J.1238.2010.00569

湖南农业大学青年科学基金项目（08QN22）；

本校为第一机构

建立了一种测定饲料及饲料原料中黄曲霉毒素B1,B2,G1和G2含量的光化学衍生-高效液相色谱方法.试样经甲醇与水的混合液(体积比80:20)提取,免疫亲和柱净化,浓缩,高效液相色谱分离,在线光化学柱后衍生,荧光检测器检测,外标法定量,在优化的条件下,混合标准工作液中黄曲霉毒素B1,B2,G1和G2的浓度分别为0.38~20.00μg/L时,浓度与峰面积线性关系良好,相关系数为0.9996~0.9999;在加标浓度0.75~20.00μg/kg,样品平均回收率为81.9%~98.7%,日内相对标准偏差1.57%~3.87%,日间相对标准偏差3.01%~6.87%;按信噪比3计算,黄曲霉毒素B1,B2,G1和G2的检出限分别为0.05,0.02,0.05,0.08μg/kg.该方法简便快速,灵敏度高...

A high performance liquid chromatography with post-column photochemical derivatization method has been developed for the simultaneous determination of aflatoxins B1,B2,G1 and G2 in feed and feed stuffs.The samples were extracted with methanol-water solution （80：20,V/V）,and were cleaned up by immunoaffinity columns.The separation of aflatoxins B1,B2,G1 and G2 were conducted by HPLC,the determination was carried out by fluorescence detector after photochemical derivatization and quantified by external standard method.Under the optimal conditio...