期刊论文

中文

湖南农业大学学报(自然科学版)

1007-1032

2010

36

1

73-76

湖南省科学技术厅重点项目（01NKY1002-01）； 湖南农业大学人才稳定基金项目（09WD05）；

本校为第一机构

将1日龄小鼠卵巢移植入成年受体雄鼠肾囊下,分别于移植后21 d(A组)和28 d(B组),回收卵巢移植体和卵母细胞,采集附睾尾精子,并对睾丸及精子进行形态学检测,计算畸形精子数.移植受体雄鼠的繁殖能力将依据与雌鼠合笼交配后的窝产活仔记录进行评价.结果表明,卵巢移植体生长并有卵泡发育,A、B组回收卵母细胞数组间无显著差异(P>0.05);移植组不同时期卵巢移植体对雄鼠睾丸及精子形态无明显影响,其畸形率与对照组(正常雄鼠)相比差异不显著(P>0.05);移植受体雄鼠与母鼠配种的窝产活仔数与对照组无显著差异(P>0.05).初步证实在卵巢异性移植构建雌、雄性腺同体的生理环境中,卵巢移植体对受体雄鼠精子及生殖...

Effects of ovarian transplantation on sperms and reproductive ability of male recipient mice were investigated by designing group A, B for ovarian transplantation and group contorl. 1-day-old mouse ovaries were transplanted into kidney capsule of adult male mice and collected to observe their status of development,recovere oocytes were recovered from ovarian groofts on day 21 and 28 after the transplantation in groups A and B respectively. At the same time, the testes and sperms from epididymies of male recipients were observed morphologically ...