期刊论文

Study on extraction of 3-30 kD antimicrocial peptides and its antibacterial activity

中文

湖南农业大学学报(自然科学版)

1007-1032

2010

S1

50-53

本校为第一机构

从蚯蚓体内分离纯化30 kD以下的抗菌肽,并研究其抑菌效果.通过电击诱导蚯蚓产生抗菌肽、超滤浓缩脱盐以及截取3～30 kD的抗菌肽、用阳离子交换分离除去较多杂蛋白、凝胶过滤层析进一步精确分离提纯得到3~30 kD的蚯蚓抗菌肽,有H1和H2两个蛋白浓度较高的峰值.测得其蛋白质量浓度分别为6.82、7.46 mg/μL,其相对分子质量分别在5 856～7 823 kD和14 40～20 00.抑菌活性测定结果表明,H1和H2都有较好的抑菌效果,其中H1对草枯杆菌、金黄色葡萄球菌、巴氏杆菌、大肠杆菌有明显的抑菌效果,H2对巴氏杆菌、大肠杆菌和草枯杆菌也有明显的抑菌效果,H1的抑菌活性比H2高.

In order to isolate and purify the 3 ~30 kD antimicrobial peptides from earthworm and examine its antibacterial activity.The antimicrobial peptide of earthworm were produced by lightning stroke,and isolate the 3~30 kD antimicrobial peptides by ultrafiltrating,concentration and desalinization.Using the SP-Sepharose Fast Flow ion exchang chromatography to remove most of the hybridprotein and Sephadex G-50 gel filtration chromatography to precisely abstracte 3~30 kD antimicrobial peptides which have two wave crest of protein named H1,H2.Their prot...