期刊论文

中文

湖南农业大学学报(自然科学版)

1007-1032

2010

36

5

574-580

10.3724/SP.J.1238.2010.00574

湖南省教育厅项目（08C433）； 湖南省研究生创新项目（14）；

本校为第一机构

为大量获得高纯度的桦木酸及准确测定桦木酸的含量,从白桦树皮中提取桦木醇,经琼斯试剂氧化制备中间产物桦木酮酸后,用硼氢化钠还原合成桦木酸,采用IR、HPLC-MS、1H-NMR以及13C-NMR对桦木醇和桦木酸的结构进行鉴定,确定最佳提取与合成条件,并建立了HPLC测定桦木酸含量的方法:色谱柱Zorbax Eclipse XDB-C8(4.6 mm×150 mm,5 μm);流动相∶甲醇-水(4 g/L甲酸铵)=40∶60,流速1.0 mL/min, 波长210 nm,柱温为室温.结果表明,桦木醇最佳提取条件:甲醇为溶剂,固液比为15/200(g/mL),70 ℃,提取3 h;桦木酸最佳合成条件为:桦木醇与琼斯试剂的摩尔比为1∶6,20 ℃反应3 h.桦木酸的线性范围为0.031 25～0.5 mg...

In order to get plenty of betulinic acid with high purity and test the content of betulinic acid,betulinic acid was synthesized by oxidizing betulin from the bark of the white birth with Jone＇s reagent,followed by selective oxidation of the resulting betulonic acid with NaBH4.IR,HPLC-MS,1H-NMR and 13C-NMR were used to identify the structure of betulin and betulinic acid.To compare extraction efficiency,several solvents were evaluated,also extraction time and extraction time were optimized.A quantitative analysis method of betulinic acid by HPL...