期刊论文

中文

湖南农业大学学报(自然科学版)

1007-1032

2011

37

2

211-214

10.3724/SP.J.1238.2011.00211

(湘财教指[2009]172号):湖南省财政厅科技专项

本校为第一机构

运用本体聚合法制备L-酪氨酸分子印迹聚合物,并考察了聚合物的性能。在单因素试验考察功能单体的种类及用量、交联剂的种类及用量、溶剂种类和反应温度对制备分子印迹聚合物影响的基础上,进一步以3水平4因素的正交试验优化反应条件。利用傅立叶红外光谱分析聚合物的结构,通过荧光分光光度法分析聚合物对L-酪氨酸的选择性吸附、萃取效率和饱和吸附量。结果表明:在氯仿溶剂中,以α-甲基丙烯酸（MAA）为功能单体,二甲基丙烯酸乙二醇酯（EDMA）为交联剂,偶氮二异丁晴（AIBN）为引发剂,其物质的量比为10∶40∶200∶1.5,60℃水浴加热,洗脱剂萃取后,得到对L-酪氨酸分子有选择性吸附性能的印迹聚合物。

This paper is an attempt to study the method of preparing the L-Tyr imprinted polymer by bulk polymerization,and investigated the property of the polymer.Based on the studying of the type and amount of functional monomer,crosslinking agent and the solvent and temperature which affected the preparation of molecularly imprinted polymer,further study was made to optimize the reaction conditions by doing orthogonal experiments of four factors in three levels.The structure of the polymer was analysed by fourier infrared spectrum;The selective adsorp...