期刊论文

中文

湖南农业大学学报(自然科学版)

1007-1032

2011

37

4

456-460

10.3724/SP.J.1238.2011.00456

973;2009CB226108:国家重点基础研究发展计划（973计划） 10KFXM02:作物种质创新与资源利用国家重点实验室培育基地重点项目 10QN01:湖南农业大学青年科学基金

本校为第一机构

为获得高效利用木糖生产乙醇的菌株,以木糖为唯一碳源,从腐败的落叶层土壤采样进行分离筛选,获得1株可发酵木糖生产乙醇的细菌,初步鉴定其为阴沟肠杆菌(Enterobactercloacae),并对该菌株发酵木糖生产乙醇的工艺条件进行了优化。结果表明:在微氧、发酵温度35℃、接种量6%、初始ph值65的条件下,用该菌株发酵20g/L木糖48h的乙醇产量可达4.95g/L,乙醇得率为其理论值的53.8%;将10g/L木糖和10g/L葡萄糖混合发酵48h的乙醇产量达到4.41g/L,乙醇得率为其理论值的45.5%,表明该菌株具有较强的同时发酵木糖和葡萄糖的能力。

To obtain strains for efficient xylose fermentation, a bacteria strain which can convert xylose to ethanol was isolated from decomposed leaves with xylose as sole carbon substrate and was identified as Enterobacter cloacae. The process for converting xylose to ethanol was optimized. The yield of ethanol was 4.95 g/L which accounted for 53.8% of the yield predicted theoretically when Enterobacter cloacae was inoculated at a 6：100 ratio and fermented in microaerophilic condition at 35 ~C with initial pH 6.5 for 48 h using xylose（20 g/L） as sol...