期刊论文

中文

湖南农业大学学报(自然科学版)

1007-1032

2011

37

1

97-98

10.3724/SP.J.1238.2011.00097

国家科技部国际合作项目（2009DFA32350）；

本校为第一机构

采用Folin-Ciocalteu法对荷叶、枳实和水皂角3种中草药提取物的多酚定量,用分光光度法进行清除自由基能力的评估。结果表明,经过粗提后,多酚含量最高的是枳实提取物,多酚含量为（462.5±19.28）mg/g,其次是荷叶和水皂角提取物,分别含多酚（293.8±78.57）和（270.3±28.53）mg/g。清除DPPH自由基能力最强的是水皂角提取物,其次是荷叶和枳实提取物,IC50值分别为（0.021±0.003）、（0.037±0.007）和（0.061±0.010）mg/mL。抗氧化性测定结果显示,3种中草药的抗氧化性与其酚类物质含量并不呈正相关。

Folin-Ciocalteu and spectrophotometer were adopted to analyse the polyphenol content and DPPH scavenging activityof 3 kinds of extracts from Chinese herbal medicine plants.The results showed that the extract of Citrus aurantum had highest polyphenol contents,which contained（462.5±19.28） mg/g;the second was the extract of lotus leaf and the third was the extract of Cassia normame,containing（293.8±78.57） mg/g and（270.3±28.53） mg/g.The extract of Cassia normame had highest DPPH scavenging activity.The second and the third were the extract...