期刊论文

中文

湖南农业大学学报(自然科学版)

1007-1032

2012

38

3

300-304

10.3724/SP.J.1238.2012.00300

31101774,309972099:国家自然科学基金 2009FJ:湖南省科技计划 CCARS-37-04B:现代农业产业体系项目

本校为第一机构

为探讨双拷贝抑制素pcISI基因疫苗纯度和剂量对肉牛黄体发育的影响,将150头同期发情的南阳黄牛随机分为8个试验组和2个对照组,试验组用pcISI按2种纯度、4种剂量（0.75、1.50、2.25、3.00 mg/头）进行免疫处理,2个对照组分别用空质粒和生理盐水进行处理;初次免疫21 d后进行加强免疫。结果表明:无论用高纯度还是低纯度疫苗免疫,2.25 mg/头剂量处理对黄体的作用效果最明显,且抗体水平与黄体发育密切相关,2.25 mg/头抑制素融合表达质粒组的黄体显著大于0.75 mg/头组（P

Estrus synchronized yellow cattle （n=150） were divided into 8 experimental groups immunized with different dosages （0.75, 1.50, 2.25, 3.00 mg per animal） of highly- or lowly-purified inhibin fusion expressing plasmid pcISI and 2 control groups immunized with plasmid pcISI or physiological saline to investigate the effect of purity and dosage on corpus luteum development of yellow cattle immunized with inhibin gene expressing plasmid pcISI. Booster immunization was done 21 d after the primary immunization. The results showed that regardless ...