The coding region of a cloned cDNA of Ramie auxin binding protein gene （BnABP1） was inserted into a plant expression vector pCAMBIA1300-GFP under the control of promoter CaMV35S to construct a green fluorescent protein （GFP） fusion expression vector. The recombinant plasmid named pCAMBIA13OO-GFP-BnABP1 was transformed into Nicotiana tabacum WS38 via Agrobacterium tumefaciens mediated transformation and transgenic tobacco was obtained by screening on antibiotic media and by PCR identification. The transgenic tobacco callus was detected under...
