期刊论文

中文

湖南农业大学学报(自然科学版)

1007-1032

2012

38

3

305-309

10.3724/SP.J.1238.2012.00305

(K0902007-21):长沙市科技计划重大专项

本校为第一机构

采用小鼠体内试验和体外试验,利用Marc-145细胞体外培养系统,通过观察细胞病变来评价茶叶水提物对猪繁殖与呼吸综合征病毒(PRRSV)繁殖的抑制作用.改变加入茶叶水提取物的方式(茶叶水提物先与病毒感作后接种细胞、茶叶水提物与已感染病毒的细胞作用2种方式),初步探讨茶叶水提物对PRRSV的抑制与杀灭作用.体内试验是采用小鼠尾静脉注射的方法,将茶叶水提物注射到小鼠体内,测定小鼠血清对PRRSV的作用.结果表明,茶叶水提物对PRRSV具有杀灭与抑制作用,碧螺春茶(不发酵)、生普洱茶(不发酵)、铁观音茶(半发酵)、熟普洱茶(后发酵)的水提物对PRRSV的最小抑制质量浓度分别为31.3、31.3、31.3、125 μg/mL,最...

Based on both the in vivo and in vitro way and the Marc 145 cell culture system, the effects of aqueous extracts from teas on porcine reproductive and respiratory syndrome （PRRS） was assessed regarding the cytopathic effect （CPE）. First, the antiviral effect of the aqueous extracts was investigated by infecting the cell after the virus reacted with tile aqueous extracts and by simultaneously adding the virus and the aqueous extracts to the cells. Then, the in vivo experiment was conducted by measuring the anti-PRRSV effect of sera collected...