期刊论文

中文

湖南农业大学学报(自然科学版)

1007-1032

2012

38

3

256-261

10.3724/SP.J.1238.2012.00256

30871336,31000536:国家自然科学基金 2011M500963:中国博士后科学基金 09JJ3043:湖南省自然科学基金重点项目 2011RS4009:湖南省博士后科研专项 09A038:湖南省教育厅重点项目 :湖南省优秀博士学位论文获奖作者科研项目

本校为第一机构

利用数据库资源和RT–PCR技术获得烟草WRKY8基因,序列分析表明,该基因包含1 551 bp的开放阅读框,编码516个氨基酸。基因编码的蛋白含有2个WRKY结构域,其锌指结构类型为C2H2(C–X_(4–5)–C–X(_22–23)–H–X_1–H)。推测其可能定位于细胞核并具有复制转录及调控的功能,从而参与对植物生长发育和胁迫应答的调节。RT–PCR结果表明,NtWRKY8基因受低温诱导表达。

In this paper, the NtWRKY8 gene was cloned from Nicotiana tabacum L. based on the database resources and RT-PCR technology. Sequence analysis showed that NtWRKY8 gene contained a complete ORF, which was 1 551 bp and encoded a protein of 516 amino acid residues. The WRKY8 protein had two conserved WRKY domains, and the zinc-finger structure was C2H2 （C-X4_5-C-X22_23-H-X1-H）. Functional analysis indicated that this protein was probably located in the nucleus and had functions in replication, transcription and regulatory, and consequently could ...