期刊论文

中文

湖南农业大学学报(自然科学版)

1007-1032

2012

38

5

519-525

10.3724/SP.J.1238.2012.00519

IRT0963:教育部***和创新团队发展计划项目 2011FJ3040:湖南省科技计划

本校为第一机构

采用酶消化法原代培养大鼠肠平滑肌细胞,用倒置相差显微镜测定血根碱对大鼠肠平滑肌细胞收缩的影响。结果表明:酶消化法原代培养的大鼠肠平滑肌细胞纯度高（α–actin检测阳性率达90%以上）,密度大,并呈现平滑肌细胞特有的"峰–谷"样排列;血根碱及阿托品可显著抑制肠平滑肌细胞收缩,抑制率分别为32.22%和34.99%;血根碱和阿托品联用对肠平滑肌细胞收缩的抑制率可达68.8%,与单独使用血根碱或阿托品的抑制率差异显著;血根碱对乙酰胆碱、组胺及KCl诱导的肠平滑肌细胞的收缩具有显著的抑制作用;血根碱主要通过作用于细胞膜上的M受体、H1受体和钙离子通道来实现对大鼠肠平滑肌细胞收缩的抑制作用。

The influence of sanguinarine（SA） on contraction of smooth muscle cells isolated from rat intestines using collagenase digestion was measured by inverted phase–contrast microscope.The result showed that rat intestinal smooth muscle cells isolated by enzyme digestion showed high purity,high density and ＂peak–valley＂ arrangement which is unique to smooth muscle cells.SA and atropine showed significant inhibition on the contractions of the isolated cells,the inhibition rates were 32.22% and 34.99% respectively.The inhibition rate of SA toget...