A Pyrazosulfuronethyl-degrading strain DF 12 was isolated from sewage reservoir of the herbicides factory in Hunan, which belonged to Pseudomonas putida based on physiological and biochemical properties and 16S rDNA analysis. When strain DF12 grown in LB medium was adjusted to an 00600 of 0.80 and inoculated in an inoculum size of 2.0% in 70 mL culture containing Pyrazosulfiaronethyl in a 100 mL flask with shaking under pH of 7.0,...