To reveal the characteristics of the mrf4 gene in the mandarin fish and analyze the expression pattern ofmrf4 in different tissues of the adult mandarin fish and in embryos from different developing stages. Reverse transcriptional PCR was used to amplify the mrf4 eDNA sequence of mandarin fish, which was inserted into a cloning vector and sequenced. Quantitative real-time PCR was conducted on samples from different tissues of adult mandarin fish and from embryos of different stages. The results show that the mrf4 gene of mandarin fish is 726 bp...
