期刊论文

中文

湖南农业大学学报(自然科学版)

1007-1032

2013

39

3

265-269

10.3724/SP.J.1238.2013.00265

基金项目：国家“973”项目（2012CB723004） 国家农业科技成果转化项目（2012GB2D200328）

本校为第一机构

为验证Lacc1基因的功能,揭示灵芝漆酶基因及其启动子结构与其转录表达的内在关系,预测了Lacc1的氨基酸序列结构及灵芝漆酶注释基因Lacc1的上游启动子区域,研究已测序的菌株——灵芝P9在Cu~(2+)诱导条件下漆酶基因Lacc1的表达情况。Protein Blast分析结果表明,Lacc1含有3个铜氧化酶(Cu-oxidase)保守结构域,与Polyporus ciliatus漆酶lcc3-3的相似性最高,为71%,并具有高度保守的漆酶特征序列L1-L4;亚细胞位置和信号肽预测结果表明,Lacc1含有信号肽,且为胞外分泌酶;Lacc1基因上游启动子区域包含1个TATAbox、3个金属响应元件(MRE)、5个氮因子结合位点(NIT2)、1个压力响应元件(STRE);在150μmol/L Cu~...

To verify the function ofLaccl gene and investigate the relationship between the gene, promoter structure and transcription, expression for Ganoderma lucidum Laccase, we analyzed the amino acid structure of Laccl and upstream promoter region of putative laccase gene Laccl, whilst studied the putative laccase gene transcription in Ganoderma lucidum strain P9 induced by Cu2＋. Result of protein blast showed that Laccl had three Cu-oxidase conserved domains and multiple sequence alignments showed that Lacc 1 had the highest homology with lcc3-3 of...