期刊论文

Molecular level's evaluation on the genetic diversity of pummelo germplasm and their relative species in hunan province based on srap markers

中文

湖南农业大学学报(自然科学版)

1007-1032

2016

42

6

608-614

10.13331/j.cnki.jhau.2016.06.005

湖南省农业科学院科技创新团队项目(2014td04)； 中南大学博士创新基金项目(2340–77226)；

本校为其他机构

以湖南本地柚类资源为试材,应用SRAP标记对41份柚类资源及5份近缘种的遗传多样性进行分析与鉴定。结果表明:平均每个引物组合可扩增出16.6条谱带,17对SRAP引物共扩增出283条谱带,其中多态性谱带235条,多态率为83.0%,基因多样度变幅为0.431 7~0.770 0,平均基因多样度为0.544 3;获得了21个基因型的37个SRAP特异性标记,不同引物组合可将42个基因型完全分开;柚类及近缘种等位基因平均数、平均杂合位点占比、SRAP表型杂合度(H_0)分别为9.02、67.77%和0.343。聚类分析结果显示,41份柚种质及5份近缘种材料在遗传相似系数0.792处可分为6个组群:第1、2组群分别由24个和11个柚的地方品种构成;第3组群为柚的...

Genetic diversity of 41 pummelo (Citrus grandis Osbeck) germplasms and 5 relative species collected from Hunan province were identified and evaluated using the markers from sequence related amplified polymorphism (SRAP). The results showed that the average number of 16.6 alleles could be amplified out from every primer combination, and total of 283 alleles were detected from 17 SRAP markers, 235 out of which presented polymorphic, accounted for 83.0%. Gene diversity value changed from 0.431 7 to 0.770 0 with an average of 0.544 3 for each primer, which showed a high level of genetic diversity ...