期刊论文、会议论文

中文

湖南农业大学学报(自然科学版)

1007-1032

2016

42

6

592-600

2016湖南茶业科技创新论坛

2016湖南茶业科技创新论坛论文集

2016-12-01

长沙

湖南省茶叶学会

10.13331/j.cnki.jhau.2016.06.003

31571802:国家自然科学基金 14A066:湖南省教育厅重点项目

本校为第一机构

为探明不同地域茯砖茶中“金花”菌的异同,采用陕西、湖南和浙江3个产区同等级的黑毛茶为原料,分别在本地及其他2个产区同一时期加工成9个茯砖茶样,运用纯培养技术,观察茶样中“金花”菌在不同培养基上的形态特征,同时结合现代分子生物学技术,对茶样中的“金花”菌进行了鉴定。结果表明:从9个茯砖茶样品中,分离获得了13株“金花”菌,在不同培养基中的形态学特征与散囊菌属的特征非常相近,且菌株G9、G10的形态特征与其他“金花”菌明显不同;通过对特异性区段ITS和β–tubulin序列扩增、测序,经同源序列搜索比对及构建的系统发育树进一步分析,并参照Hubka最新的曲霉属分类系统,最终将不同地域(陕、...

In order to study the differences of the “Jinhua” fungi from the fuzhuan tea (FZT)produced in different regions, 9 FZT samples, which were processed with the same standard of raw dark teas from Shanxi, Hunan, Zhejiang at the same season, were used as materials. The “Jinhua” fungi obtained from the FZT samples were cultured with different mediums. The species of the tested fungi were identified by the morphology of colony observed with microscope and DNA sequencing. The results revealed that 13 strains of “Jinhua” fungi were obtained from ...