期刊论文

中文

湖南农业大学学报(自然科学版)

1007-1032

2016

42

3

322-327

10.13331/j.cnki.jhau.2016.03.019

农业部农药登记残留项目

本校为第一机构

建立一种高效液相色谱法同时检测稻田土壤、稻田水和糙米中二甲四氯、2,4,5-T、高效氟吡甲禾灵、精吡氟禾草灵和精喹禾灵5种除草剂残留的方法。土壤样品用0.05%磷酸溶液和乙腈混合液(体积比为1∶2)提取,水样品用乙酸乙酯萃取,糙米样品用0.04 mol/L NaOH提取,用弗罗里硅土柱净化。采用带紫外检测器的高效液相色谱仪测定,以乙腈与水为流动相梯度洗脱,在紫外波长229 nm下检测,用外标法定量。结果表明,5种除草剂在质量浓度0.05~5.00 mg/L的线性关系良好,相关系数(R)达0.999 7~0.999 9;土壤和糙米中添加水平为0.05~ 1.00 mg/kg时,5种除草剂的平均回收率为78.8%~107.8%,相对标准偏差(RSD≤7.5, n=5)为...

To simultaneously determine residues of five herbicides, namely MCPA,2,4,5-T, haloxyfop-p-methyl,quizafol-p-thyl and fluazifop-p-butyl in paddy soil, water and coarse rice, a new method was developed using highperformance liquid acid and aetonitrile chromatography. The residues in soil was extracted with mixed solution of 0.05% ortho-phosphoric （volume ratio of 1 ： 2）, and those in water was extracted with ethyl acetate, while in coarse rice was dissolved by 0.04 mol/L sodium hydroxide aqueous, then purified by a florisil column. Five herbic...